Study of proliferative activity and gene expression (col1a1, mmp1a, mmp1b, mmp3, vcan, eln) in fibroblasts after exposure to collagen preparations

Morzhanaeva М.А., Svechnikova E.V., Babin Y.Y., Starkina O.V.

LLC «Beauty Expert Medical», Moscow, Russia
"Polyclinic No. 1" Department of presidential Affairs, Moscow, Russia
Medical Institute of Continuing Education «ROSBIOTECH», Moscow, Russia
LLC «Melsytech», genetics laboratory "Melsytech Genetics", Nizhny Novgorod, Russia

The aim of the study was to evaluate the viability and proliferative activity of fibroblasts, as well as gene expression (collagen type 1 (COL1), elastin (ELN), matrix metalproteinase type 1 (MMP1), matrix metalproteinase type 3 (MMP3) and versican (VCAN )) after exposure to six different collagen preparations - dermal fillers.
Materials and methods. The standard MTT test was used to measure cell viability, proliferation and cytotoxicity. Gene expression was measured using real-time reverse transcription polymerase chain reaction (RT-rtPCR).
Results. The results obtained showed that the effectiveness of the studied drugs does not depend on the concentration of collagen in the final solution. When performing the MTT test, the greatest proliferative activity was observed for fibroblasts incubated with the Collost micro drug. For the Linerase drug, after 48 hours, a higher expression of collagen was observed compared to other drugs. It should be noted that there was no expression of the mmp1 gene after 24 hours in all samples except those treated with Collapro30+ and Collapro55+.
Based on the results of pairwise comparison, no significant differences between groups in MMP3 gene expression (24 hours and 48 hours after incubation) were revealed. Also, in samples treated with Collapro30+, Collapro45+ and Collapro55+, versican is expressed at a higher level on the first day of the study. In turn, on the second day, versican is expressed at a higher level in the Collapro45+ and Linerase preparations.