Immunofunctional properties of cultured human mast cells and their application in IgE-mediated hypersensitivity reaction diagnosis

Romanova I.V., Hancharou A.Y., Ruban A.P., Sheremetyeva L.Z.

Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus, Minsk, Belarus
Healthcare Institution “3rd City Clinical Hospital named after E.V. Klumova”, Minsk, Belarus

A promising option for diagnosing immediate allergic reactions is the assessment of allergen-specific activation of mast cells using standardized allergens and patient serum that potentially contains IgE antibodies. We have developed a method for obtaining mast cells from umbilical cord blood-derived hematopoietic stem cells. This article reviews the role of mast cells in the development of allergic inflammation, describes the method of their generation ex vivo, their immunomorphological properties, as well as the possibility of their practical use in laboratory diagnostics of IgE-mediated reactions.